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ANALYTICAL METHOD DEVELOPMENT AND VALIDATION
FOR ESTIMATION OF GABAPENTIN IN HUMAN PLASMA
AND PHARMACEUTICAL DOSAGE FORMS
A
Thesis Submitted to
GANPAT UNIVERSITY, KHERVA
In Partial Fulfillment of the requirement for the degree of
MASTER OF PHARMACY
IN
THE FACULTY OF PHARMACY
(QUALITY ASSURANCE)
APRIL-2009
Guided By: Submitted By:
Dr. S. A. Patel DUSHYANT PATEL
M. Pharm., Ph. D. B. Pharm.
S. K. PATEL COLLEGE OF PHARMACEUTICAL EDUCATION AND RESEARCH
GANPAT UNIVERSITY, KHERVA - 382711
DIST. – MEHSANA, GUJARAT, INDIA.
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Sommario

Pagina 1 -

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF GABAPENTIN IN HUMAN PLASMA AND PHARMACEUTICAL DOSAGE FORMS A Thesis Submitted to GANP

Pagina 2 - CERTIFICATE

Dushyant Patel List of Figures S.K.P.C.P.E.R., Kherva

Pagina 3 - DECLARATION

Dushyant Patel List of Abbreviations S.K.P.C.P.E.R., Kherva

Pagina 4 - ACKNOWLEDGMENT

Dushyant Patel List of Abbreviations S.K.P.C.P.E.R., Kherva

Pagina 5

Dushyant Patel Introduction S.K.P.C.P.E.R., Kherva

Pagina 6

Dushyant Patel Introduction S.K.P.C.P.E.R., Kherva

Pagina 7 - SR NO. TITLE

Dushyant Patel Introduction S.K.P.C.P.E.R., Kherva

Pagina 8 - List of Tables

Dushyant Patel Introduction S.K.P.C.P.E.R., Kherva

Pagina 9

Dushyant Patel Introduction S.K.P.C.P.E.R., Kherva

Pagina 10 - List of Figures

Dushyant Patel Introduction S.K.P.C.P.E.R., Kherva

Pagina 11

Dushyant Patel Introduction S.K.P.C.P.E.R., Kherva

Pagina 12

This is to certify that the research work embodied in this thesi

Pagina 13 - CHAPTER 1: INTRODUCTION

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 14

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 15

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 16 - 1.2 DRUG PROFILE

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 17

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 18

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 19 - 1.3 REFERENCES:

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 20 - 2.1 REVIEW OF LITERATURE:

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 21

Dushyant Patel Review of Literature S.K.P.C.P.E.R., Kherva

Pagina 22

Dushyant Patel Aim of The Present Work S.K.P.C.P.E.R., Kherva

Pagina 23

I hereby declare that this thesis entitled “ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF GABAPENTIN IN HUMAN PLASMA AND PHAR

Pagina 24

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 25

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 26 - 2.2 REFERENCES:

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 27 -  J Pharm Biomed Anal

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 28

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 29 - 3.1 AIM OF THE PRESENT WORK:

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 30 - IN HUMAN PLASMA

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 31

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 32

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 33

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 34

` A single flower cannot make a garland or a single star can not make the beautiful shiny sky at the night, same way a research work can never b

Pagina 35

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 36

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 37

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 38

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 39

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 40

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 41

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 42

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 43 - 4.2 EXPERIMENTAL WORK:

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 44

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 45

I owe a special word of thanks to Mr. Jignesh Bhatt, Dr. Deepak Jain, Dr. Tushar Mehta and Mr. Mohan Kundlik for their profound guidance and shari

Pagina 46

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 47

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 48

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 49

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 50

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 51

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 52

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 53

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 54

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 55

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 56

Their firm and kind hearted personality has affected me to be steadfast and never bend to difficulty. They always let me know that they are proud of

Pagina 57

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 58

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 59

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 60

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 61

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 62

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 63 - 4.3 RESULTS AND DISCUSSION:

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 64

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 65

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 66

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 67

Dushyant Patel Index S.K.P.C.P.E.R., Kherva

Pagina 68

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 69

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 70

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 71

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 72

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 73

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 74

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 75

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 76

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 77

Dushyant Patel LC-MS/MS Method S.K.P.C.P.E.R., Kherva

Pagina 78 - 4.4 REFERENCES:

Dushyant Patel List of Tables S.K.P.C.P.E.R., Kherva

Pagina 79

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 80

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 81

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 82

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 83

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 84

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 85

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 86 - 5.3 RESULTS AND DISCUSSION:

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 87 - Absorbance

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 88

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 89

Dushyant Patel List of Tables S.K.P.C.P.E.R., Kherva

Pagina 90 - 5.4 REFERENCES:

Dushyant Patel Extractive Spectrophotometric Method S.K.P.C.P.E.R., Kherva

Pagina 91 - 6.2 CONCLUSION:

Dushyant Patel Summary and Conclusion S.K.P.C.P.E.R., Kherva

Pagina 92

Dushyant Patel Summary and Conclusion S.K.P.C.P.E.R., Kherva

Pagina 93 - Pharm Sci

Dushyant Patel Presentations and publications S.K.P.C.P.E.R., Kherva

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